Mass production of Metarhizium anisopliae (green muscardine fungus)

Pest managed

Rhinoceros beetle - Oryctes rhinoceros

On coconut water

Materials required

• Coconut water
• Flat glass bottles
• Cotton plug
• Pressure cooker
• Injection syringe
• Bunsen burner
• Laminar flow chamber
• Mixer grinder

Methodology

Take glass bottles containing 40 ml of coconut water. Plug these bottles with cotton plug and sterilize in autoclave for 20 minutes at 15 psi. The bottles are inoculated with 1 ml suspension containing spores of the fungus with the help of a sterile injection syringe. Sterilize the bottles with the help of burner. Spores are inoculated in bottles with the help of syringe in a laminar flow chamber. Keep inoculated bottles till the surface of medium is fully covered by the olive green sporulated fungus. Whole culture is grinded thoroughly in mixer. Keep culture in cool and dry place in packets.

In Carrot broth

Material Required

• Carrots
• Conical flask (250 ml)
• Distilled water
• Autoclave
• Laminar flow chamber
  
  

Methodology

Wash thoroughly cut pieces of carrots put in conical flask. Add 15 ml of distilled water in conical flask. Conical flasks are plugged with cotton and autoclaved for 20 min at 15 psi and allow for cooling. Inoculate mother culture in slant loopful quantities of M. anisopliae spores in flasks. The flasks are incubated at room temperature. Spores will be ready in 15 days.

Source: Skill Development of Farmers for Production of IPM inputs in IPM Seva Kendra